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| Registros recuperados : 16 | |
| 7. |  | SÁNCHEZ-NAVARRO, J. A.; FIORE, N.; FAJARDO, T. V. M.; PALLÁS, V. Simultaneous detection of the 13 viruses and 5 viroids affecting grapevine by molecular hybridization using a unique probe or 'polyprobe'. In:Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), 19., 2018, Santiago, Chile. Anais... santiago, Chile: ICGV, April, 2018. p. 40-41.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | FAJARDO, T. V. M.; PEIRÓ, A.; SÁNCHEZ-NAVARRO, J. A.; PALLÁS, V. Role of the 5' untranslated region of the Alfalfa mosaic virus RNA 3 in cell-to-cell and long distance transport. Virología, Madrid, v. 16, n. 2, p. 143-144, 2013. Extraordinario. Edição dos Resumos do 12º Congreso Nacional de Virología, Burgos, Espanha, jun. 2013.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 10. | | LEASTRO, M. O.; CASTRO, D. Y. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Citrus LEPROSIS virus c codifica três proteínas com atividades supressoras de silenciamento gênico Citrus Leprosis Virus C encodes three proteins with gene silencing suppression activity. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 52., 2021. Anais... [Brasília, DF]: Sociedade Brasileira de Fitopatologia, 2021. p. 190. Evento online.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. Viruses , v.13, n.12, 2021.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | APARICIO, F.; FAJARDO, T. V. M.; HERRANZ, M. C.; PEIRÓ, A.; PALLÁS, V.; SANCHEZ-NAVARRO, J. A. The transport of plant viruses: a critical step in the viral cycle. Tropical Plant Pathology, Brasília, DF, v. 36, 2011. p. 1312-1315. 1 CD-ROM. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Mesa redonda 10.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 14. | | FIORE, N. UNES, M. Z.; ZAMORANO, A.; FAJARDO, T. V. M.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. A. Simultaneous detection of the main viruses and viroids affecting grapevine by molecular hybridization using a unique riboprobe or 'Polyprobe'. In: CONGRESS OF THE INTERNATIONAL COUNCIL FOR THE STUDY IF VIRUS AND VIRUS-LIKE DISEASES OF THE GRAPEVINE (ICVG), 17., 2012, Davis, CA. Proceedings... Davis: UCLA, Foundation Plant Services, 2012. p. 146-147.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 15. | | KUHN, J. H.; ADKINS, S.; ALIOTO, D.; ALKHOVSKY, S. V.; AMARASINGHE, G. K.; AVSIC-ZUPANC, T.; AYLLÓN, M. A.; BAHL, J.; BALKEMA-BUSCHMANN, A.; BALLINGER, M. J.; BARTONICKA, T.; BASLER, C.; BAVARI, S.; BEER, M.; BENTE, D. A.; BERGERON, É.; BIRD, B. H.; BLAIR, C.; BLASDELL, K. R.; BRADFUTE, S. B.; BREYTA, R.; BRIESE, T.; BROWN, P. A.; BUCHHOL, U. J.; BUCHMEIER, M. J.; BUKREYEV, A.; BURT, F.; BUZKAN, N.; CALISHER, C. H.; CAO, M.; CASAS, I.; CHAMBERLAIN, O.; CHANDRAN, K.; CHARREL, R. N.; CHEN, B.; CHIUMENTI, M.; CHO, IL-R.; CLEGG, J. C. S.; CROZIER, I.; GRAÇA, J. V. da; BÓ, E. D.; DÁVILA, A. M. R.; LA TORRE, J. C. de; LAMBALLERIE, X. de; SWART, R. L. de; DI BELLO, P. L.; DI PAOLA, N.; DI SERIO, F.; DIETZGEN, R. G.; DIGIARO, M.; DOLJA, V. V.; DOLNIK, O.; DREBOT, M. A.; DREXLER, J. F.; DÜRRWALD, R.; DUFKOVA, L.; DUNDON, W. G.; DUPREX, W. P.; DYE, J. M.; EASTON, A. J.; EBIHARA, H.; ELBEAINO, T.; ERGÜNAY, K.; FERNANDES, J.; FOOK, A. R.; FORMENT, P. B. H.; FORTH, L. F.; FOUCHIER, R. A. M.; ASTUA, J. de F.; GAGO-ZACHERT, S.; GÃO, G. F.; GARCÍA, M. L.; GARCÍA-SASTRE, A.; GARRISON, A. R.; GBAKIMA, A.; GOLDSTEIN, T.; GONZALEZ, J. P. J.; GRIFFITHS, A.; GROSCHUP, M. H.; GÜNTHER, S.; GUTERRES, A.; HALL, R. A.; HAMMOND, J.; HASSAN, M.; HEPOJOKI, J.; HEPOJOKI, S.; HETZEL, U.; HEWSON, R.; HOFFMANN, B.; HONGO, S.; HÖPER, D.; HORIE, M.; HUGHES, H. R.; HYNDMAN, T. H.; JAMBAI, A.; JARDIM, R.; JIANG, D.; JIN, Q.; JONSON, G. B.; JUNGLEN, S.; KARADAG, S.; KELLER, K. E.; KLEMPA, B.; KLINGSTRÖM, J.; KOBINGER, G.; KONDO, H.; KOONIN, E. V.; KRUPOVIC, M.; KURATH, G.; KUZMIN, I. V.; LAENEN, L.; LAMB, R. T A.; LAMBERT, A. J.; LANGEVIN, S. L.; LEE, B.; LEMOS, E. R. S.; LEROY, E. M.; LI, D.; Li, J.; LIANG, M.; LIÚ, W.; LIÚ, Y.; LUKASHEVICH, I. S.; MAES, P.; SOUZA, W. M. de; MARKLEWITZ, M.; MARSHALL, S. H.; MARTELLI, G. P.; MARTIN, R. R.; MARZANO, S.-L.; MASSART, S.; MCCAULEY, J. W.; MIELKE-EHRET, N.; MINAFRA, A.; MINUTOLO, M.; MIRAZIMI, A.; MÜHLBACH, H.-P.; MÜHLBERGER, E.; NAIDU, R.; NATSUAKI, T.; NAVARRO, B.; NAVARRO, J. A.; NETESOV, S. V.; NEUMANN, G.; NOWOTNY, N.; NUNES, M. R. T.; NYLUND, A.; ØKLAND, A. L.; OLIVEIRA, R. C.; PALACIOS, G.; PALLAS, V.; PÁLYI, B.; PAPA, A.; PARRISH, C. R.; PAUVOLID-CORRÊA, A.; PAWESKA, J. T.; PAYNE, S.; PÉREZ, D. R.; PFAFF, F.; RADOSHITZKY, S. R.; RAHMAN, A.; RAMOS-GONZÁLEZ, P. L.; RESENDE, R. O.; REYES, C. A.; RIMA, B. K.; ROMANOWSKI, VÍCTOR; LUNA, G. R.; ROTA, P.; RUBBENSTROTH, D.; RUNSTADLER, J. A.; RUZEK, D.; SABANADZOVIC, S.; SALÁT, J.; SALL, A. A.; SALVATO, M. S.; SARPKAYA, K.; SASAYA, T.; SCHWEMMLE, M.; SHABBIR, M. Z.; SHÍ, X.; SHÍ, Z.; SHIRAKO, Y.; SIMMONDS, P.; SIRMAROVÁ, J. 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology, September, 2020.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | KUHN, J.; ADKINS, S.; AGWANDA, B. R.; KUBRUSLI, R. A.; ALKHOVSKY, S. V.; AMARASINGHE, G. K.; TATJANA, A.-Z.; AYLLÓN, M. A.; BAHL, J.; BALKEMA BUSCHMANN, A.; BALLINGER, M. J.; BASLER, C. F.; BAVARI, S.; BEER, M.; BEJERMAN, N.; BENNETT, A. J.; DBENTE, D. A.; BERGERON, É.; BIRD, B. H.; BLAIR, C. D.; BLASDELL, K. R.; BLYSTAD, D. R.; BOJKO, J.; BORTH, W. B.; BRADFUTE, S.; BREYTA, R.; BRIESE, T.; BROWN, P. A.; BROWN, J. K.; BUCHHOLZ, U. J.; BUCHMEIER, M. J.; ABUKREYEV, A.; BURT, F.; BÜTTNER, C.; CALISHER, C. H.; CAO, M.; CASAS, I.; CHANDRAN, K.; CHARREL, R. N.; CHENG, Q.; CHIAKI, Y.; CHIAPELLO, M.; CHOI, I. R.; CIUFO, M.; CLEGG, J. C. S.; CROZIER, I.; BÓ, E. D.; DE LA TORRE, J. C.; LAMBALLERIE, X. de; SWART, R. L. de; DEBAT, H.; DHEILLY, N. M.; DI CICCO, E.; DI PAOLA, N.; DI SERIO, F.; DIETZGEN, R. G.; DIGIARO, M.; DOLNIK, O.; DREBOT, M. A.; DREXLER, J. F.; DUNDON, W. G.; DUPREX, W. P.; DÜRRWALD, R.; DYE, J. M.; EASTON, A. J.; EBIHARA, H.; ELBEAINO, T.; ERGÜNAY, K.; FERGUSON, H. W.; FOOKS, A. R.; FORGIA, M.; FORMENTY, P. B. H.; FRÁNOVÁ, J.; ASTUA, J. de F.; FU, J.; FÜRL, S.; ZACHERT, S. G.; GĀO, G. F.; GARCÍA, M. L.; SASTRE, A. G.; GARRISON, A. R.; GASKIN, T.; GONZALE, J. P. J.; GRIFTHS, A.; GOLDBERG, T. L.; GROSCHUP, M. H.; GÜNTHER, S.; HALL, R. A.; HAMMOND, J.; HAN, O.; HEPOJOKI, J.; HEWSON, R.; HONG, J.; HONG, N.; HORIE, M.; HU, J. S.; HU, T.; HUGHES, H. R.; HÜTTNER, F.; HYNDMAN, T. H.; ILYAS, M.; JALKANEN, R.; JIĀNG, D.; JONSON, G. J.; JUNGLEN, S.; KADONO, F.; KAUKINEN, K. H.; KAWATE, M.; KLEMPA, B.; KLINGSTRÖM, J.; KOBINGER, G.; KOLONIUK, I.; KONDŌ, H.; KOONIN, V.; KRUPOVIC, M.; KUBOTA, K.; KURATH, G.; LAENEN, L.; LAMBERT, A. J.; LANGEVIN, S. L.; LEE, B.; LEFKOWITZ, E. J.; LI, L.; LEROY, E. M.; LI, S.; LÍ, J.; LIU, H.; LUKASHEVICH, I. S.; MAES, P.; SOUZA, W. M. de; MARKLEWITZ, M.; MARSHALL, S. H.; MARZANO, S. L.; MASSART, S.; MCCAULEY, J. W.; MELZER, M.; MILLER, K. M.; MING, T. J.; MIRAZIMI, A.; MORDECAI, G. J.; MÜHLBACH, H. P.; MÜHLBERGER, E.; NAIDU, R.; NATSUAKI, T.; NAVARRO, J. A.; NETESOV, S. Y V.; NEUMANN, G.; NOWOTNY, N.; NUNES, M. R. T.; VELARDE, A. O.; PALACIOS, G.; PALLÁS, V.; PÁLYI, B.; PAPA, A.; PARASKEVOPOULOU, S.; PARK, A. C.; PARRISH, C. R.; PATTERSON, D. A.; CORRÊA, A. P.; PAWESKA, J. T.; PAYNE, S.; PERACCHIO, C.; PÉREZ, D. R.; POSTLER, T. S.; QI, L.; RADOSHITZKY, S. R.; RESENDE, R. O.; REYES, C. A.; RIMA, B. K.; LUNA, G. R.; ROMANOWSKI, V.; ROTA, P.; RUBBENSTROTH, D.; RUBINO, L.; RUNSTADLER, J. A.; SABANADZOVIC, S.; SALL, A. A.; SALVATO, M. S.; SANG, R.; SASAYA, T.; SCHULZE, A. D.; SCHWEMMLE, M.; SHI, M.; SHÍ, X.; SHIMOMOTO, Y.; SHIRAKO, Y.; SIDDELL, S. G.; SIMMONDS, P.; SIRONI, M.; SMAGGHE, G.; SMITHER, S.; SONG, J. W.; SPANN, K.; SPENGLER, J. R.; STENGLEIN, M. D.; STONE, D. M.; SUGANO, J.; TABATA, A.; TAKADA, A.; TAKEUCHI, S.; TCHOUASSI, D. P.; TEFER, A.; TESH, R. B.; THORNBURG, N. J.; TOMITAKA, Y.; TOMONAGA, K.; TORDO, N.; TORTO, B.; TOWNER, J. S.; TSUDA, S.; TU, C.; TURINA, M.; TZANETAKIS, I. E.; UCHIDA, J.; USUGI, T.; VAIRA, A. M.; VALLINO, M.; VAN DEN HOOGEN, B.; VARSANI, A.; VASILAKIS, N.; VERBEEK, M.; BARGEN, S. V.; WADA, J.; WAHL, V.; WALKER, P. J.; WANG, L. F.; WANG, G.; WANG, Y.; WANG, Y.; WAQAS, M.; WÈI, T.; WEN, S.; WHITFELD, A. E.; WILLIAMS, J. V.; WOLF, Y. I.; WU, J.; XU, L.; YANAGISAWA, H.; YANG, C.; YANG, Z.; ZERBINI, F. M.; ZHAI, L.; ZHANG, Y. Z.; ZHANG, O.; ZHANG, J.; ZHANG, Z.; ZHOU, X. 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirale. Archives of Virology, August, 2021.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 16 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/08/2020 |
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Data da última atualização: |
25/08/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LEASTRO, M. O.; CASTRO, D. Y. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
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Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico; DEIBIS YORLENIS ORTEGA CASTRO, Universidad Politécnica de Valencia; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, Universidad Politécnica de Valencia. |
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Título: |
Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Microbiology, June 2020. |
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ISSN: |
1664-302X |
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DOI: |
https://doi.org/10.3389/fmicb.2020.01231 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the family Kitaviridae. MenosCitrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting th... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Categoria do assunto: |
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Marc: |
LEADER 02381naa a2200229 a 4500
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024 7 $ahttps://doi.org/10.3389/fmicb.2020.01231$2DOI
100 1 $aLEASTRO, M. O.
245 $aCitrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity.$h[electronic resource]
260 $c2020
520 $aCitrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the family Kitaviridae.
650 $aDoença de Planta
650 $aFruta Cítrica
700 1 $aCASTRO, D. Y. O.
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
700 1 $aPALLÁS, V.
700 1 $aSÁNCHEZ-NAVARRO, J. Á.
773 $tFrontiers in Microbiology, June 2020.
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